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Mechanisms of CVD and CKD in patients with diabetes Vascular damages of ASCVD in patients with diabetes are characterized by endothelial and vascular smooth muscle cell dysfunction [12]

They demonstrated that human pancreatic tumor and stellate cells activate/process GSAO into its active metabolite and that GT activity positively correlates with GSAO-mediated proliferation arrest of co-cultured endothelial cells ( Figure 3B )

Incubate cells at 4 C for 10 min followed by centrifugation for 10 min at 14,000 rpm and 4 C