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The physiological functions regulated by nitric oxide include metabolism, muscle contractility, neurological functions, the immune system, and others

Can Med Assoc J 1967

Articular cartilage and biomechanical properties of the long bones in Frzb-knockout mice

After cooling and PBS rinsing, sections were blocked with 5% BSA (30 min, RT), incubated with primary antibodies (4 C overnight), rewarmed, and washed