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The bacteria culture suspension with G2 was centrifuged at 8,000 rpm for 10 min, and it was washed in sterile distilled water, and the optical density (600 nm) of the bacterial strain was adjusted to 1 (10 8 cfu ml 1 ) using sterile distilled water

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During critical illness, muscle concentrations of GSH decrease and a change in the redox status occurs, indicative of an elevated GSSG [24]